Assessing the Protective Effects of Erythrina Senegalensis Leaf Extract on Hepatocytes and Diabetes in Albino Wistar Rats after Induction of Alloxan
Keywords:
E. senegalensis, diabetes, hepatic tissues, histopathology, liver-enzymesAbstract
The protective effect of Erythrina senegalensis leaf extract on albino Wistar rats induced with alloxan was investigated. Methanol was used as an extraction solvent. A single dose of alloxan at 150 mg/kg body weight in 0.1 M sodium citrate buffer and pH 4.5 was used to induce diabetes after an overnight fast, and blood glucose concentration was determined after 24 hours. Group 6, normal rats (non-alloxan-induced), was used as a control, and the other alloxan-induced groups were treated with E. senegalensis. They were administered 0.5 ml of distilled water twice daily for 14 days. The doses administered were 100, 150, and 200 mg/kg body weight (i.e., for groups II, III, and IV, respectively). At the end of the experimental treatments, the animals were sacrificed. Whole blood obtained by cardiac puncture from each animal was allowed to stand for 60 minutes in EDTA tubes to clot before being centrifuged at 300 x g for 10 minutes. The serum was extracted and used for enzyme assays, while their liver tissues were collected and stored in 10 % buffered formalin for histopathological examination. The AST activity of E. senegalensis leaf extract-treated animals did not significantly vary from the control at p < 0.05. The data similarly indicate no significant change in the ALT activity of the various doses between the treated rats and the control. The serum AST and ALT (De Ritis) ratios were compared. The ratio for all the treated groups did not indicate a significant variation from that of the control at p < 0.05. The results provide experimental evidence at the biochemical level showing the E. senegalensis leaf extract is non-toxic.
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